Janet L. Crosby Laganière
85 Chemin de l’Ile
Ile Cadieux, Québec
Tel: (450) 455-6648
1976 University of Connecticut, Storrs, Conn. USA
Bachelor of Science Degree in Animal Pathology (Natural Resources)
Investigation of Special Topic: “Zoonoses” – a four month independent research study done as final project
1973 Clark University and Worcester Junior College, Worcester, Mass. USA
Associate’s Degree in Sciences
1972 Konst och Miljö Skolan, Sundsvall, Sweden
Fine Arts Degree (2 yr. Undergraduate)
2013-2015 – C.R. C.H.U.M. – Centre de Recherche du Centre Hopitalier de l’Université de Montréal, Montreal, Quebec, Canada
Research Assistant for Dr. Alexandre Prat in 2- Photon Microscopy and Neuroimmunology
Skills and Duties: Research assistant in charge of a new 2- photon microscope set up for intravital microscopy. Received training at the N.I.H. in Bethesda, Maryland for thin skull animal surgery and correlating 2 photon microscopy. Helped to set up the facility and train new users in the surgery and microscopy. Carried out many immunological experiments involving cytokine detection in diseased human tissue. Carried out histological preparation of samples as well.
2005 -2013 – C.H.U.M. - Centre d’étude des maladies du cerveau (Centre for the Study of Brain Disease) University of Montreal at Notre Dame Hospital, Montreal, Quebec, Canada
1995-2005 – M.U.H.C. McGill University & Montreal General Hospital Research Institute, Montréal, Québec, Canada
Research Assistant for Dr. Guy Rouleau in Neurogenetics
Skills and Duties: Research assistant in charge of animal preparation, histology, microscopy and immuno- histo- chemistry. Also responsible for antibody production and testing. Has been involved in several independent studies including: an epilepsy seizure study, and a surgically induced stroke model in mice. Has been involved with experimentation using transgenic Zebrafish and Drosophila
Was in charge of establishing an image and microscopy centre for the Centre for Research in Brain Disease at Notre Dame Hospital. As well, was the coordinator of the CRN (Center for Research in Neurology) “Image Centre” for Montreal General Hospital for several years.
Since 1999, has been a member of the Health and Safety Committee of the MGHRI and was 12th floor monitor and representative trained in CPR.
Has been supply center manager (within the Research Institute) for the Invitrogen/Gibco Company since 1997.
1979-1995 Montreal General Hospital Research Institute, Montreal, Quebec
Laboratory technician for Dr. Michael Rasminsky in Neurophysiology, working in collaboration with Dr. A Aguayo and Dr.G.Bray.
Skills and Duties: electrophysiology, electrode manufacture, photography, microscopy. Also responsible for histology, animal preparation, surgery and dissection, immunohistochemistry and camera lucida
Independent project: 2 yrs “C-Fos activation in superior colliculus via optic nerve grafts in Golden Hamsters” involved nerve grafting, live experimentation, and immunodetection studies.
1977-1979 Cole’s Book Stores, Les Terrasses branch, Montréal, Québec
Skills and Duties: Manager, full store operation: finances, personnel, administration and sales.
Other: Over many years, has done translation and language tutoring.
English – native language;
French and Swedish – speak fluently and read/write with proficiency;
German – speak, read, and write with good competence.
West Island Youth Symphony Orchestra, Pointe Claire , Quebec
Executive Board Member from 1999-2002
Le Nichoir Bird Refuge, Hudson, Quebec
INTERESTS AND ACTIVITIES
Enjoys classical music and the arts. Since childhood, has been an avid artist and sells commercially. Also an active naturalist, with the goal of contributing to the preservation of the ecological stability in the nearby environment
Dr. Alexandre Prat, MD PhD, Principle Investigator, Neuroimmunology, Université de Montréal
Hôpital Notre-Dame – CHUM 1560 Sherbrooke Street East, Montréal,
(514) 890-8000 (24734)
Dr. Roland Auer, MD PhD FRCPC, Neuropathologist and Professor, University of Saskatchewan, Saskatoon, Saskatchewan
Dr. Aida Abu-Baker, PhD, faculty professor, Concordia University, Montreal, Quebec ; Research scientist , McGill University, Montreal Quebec, (514) 998-1646
Dr. Guy Rouleau, Principle Investigator and Director MNI McGill University, Montreal, Quebec (514) 398-1931
Dr. Michael Rasminsky, Neurologist, MUHC Montreal General Hospital
Dr. Samuel David, Researcher, Neurology, MUHC, Montreal General Hospital
(514) 934-1934 *44506
Kahle, K. T., Schmouth, J. F., Lavastre, V., Latremoliere, A., Zhang, J., Andrews, N., . . . Rouleau, G. A.
Inhibition of the kinase WNK1/HSN2 ameliorates neuropathic pain by restoring GABA inhibition.
Sci Signal (2016) 9(421), ra32. doi: 10.1126/scisignal.aad0163.
Abu-Baker A, Laganiere J, Gaudet R, Rochefort D, Brais B, Neri C, Dion PA, Rouleau GA.
Lithium chloride attenuates cell death in oculopharyngeal muscular dystrophy by perturbing Wnt/ß-catenin pathway.
Cell Death Dis. 2013 Oct 3; 4:e821. doi: 10.1038/cddis.2013.342.
Salin-Cantegrel A, Shekarabi M, Rasheed S, Charron FM, Laganière J, Gaudet R, Dion PA, Lapointe JY, Rouleau GA.
Potassium-chloride cotransporter 3 interacts with Vav2 to synchronize the cell volume decrease response with cell protrusion dynamics.
PLoS One. 2013 May 28;8(5):e65294. doi: 10.1371/journal.pone.0065294. Print 2013.
Shekarabi M, Lafrenière RG, Gaudet R, Laganière J, Marcinkiewicz MM, Dion PA, Rouleau GA.
Comparative analysis of the expression profile of Wnk1 and Wnk1/Hsn2 splice variants in developing and adult mouse tissues.
PLoS One. 2013;8(2):e57807. doi: 10.1371/journal.pone.0057807. Epub 2013 Feb 25.
Shekarabi M, Moldrich RX, Rasheed S, Salin-Cantegrel A, Laganière J, Rochefort D, Hince P, Huot K, Gaudet R, Kurniawan N, Sotocinal SG, Ritchie J, Dion PA, Mogil JS, Richards LJ, Rouleau GA.
Loss of neuronal potassium/chloride cotransporter 3 (KCC3) is responsible for the degenerative phenotype in a conditional mouse model of hereditary motor and sensory neuropathy associated with agenesis of the corpus callosum.
J Neurosci. 2012 Mar 14; 32(11):3865-76. doi: 10.1523/JNEUROSCI.3679-11.2012.
Stochmanski SJ, Therrien M, Laganière J, Rochefort D, Laurent S, Karemera L, Gaudet R, Vyboh K, Van Meyel DJ, Di Cristo G, Dion PA, Gaspar C, Rouleau GA.
Expanded ATXN3 frameshifting events are toxic in Drosophila and mammalian neuron models.
Hum Mol Genet. 2012 May 15; 21(10):2211-8. doi: 10.1093/hmg/dds036. Epub 2012 Feb 14.
Shekarabi M, Salin-Cantegrel A, Laganière J, Gaudet R, Dion P, Rouleau GA.
Cellular expression of the K+-Cl- cotransporter KCC3 in the central nervous system of mouse.
Brain Res. 2011 Feb 16; 1374:15-26. doi: 10.1016/j.brainres.2010.12.010. Epub 2010 Dec 11.
Salin-Cantegrel A, Shekarabi M, Holbert S, Dion P, Rochefort D, Laganière J, Dacal S, Hince P, Karemera L, Gaspar C, Lapointe JY, Rouleau GA.
HMSN/ACC truncation mutations disrupt brain-type creatine kinase-dependant activation of K+/Cl- co-transporter 3.
Hum Mol Genet. 2008 Sep 1; 17(17):2703-11. doi: 10.1093/hmg/ddn172. Epub 2008 Jun 19.
Shekarabi M, Girard N, Rivière JB, Dion P, Houle M, Toulouse A, Lafrenière RG, Vercauteren F, Hince P, Laganiere J, Rochefort D, Faivre L, Samuels M, Rouleau GA.
Mutations in the nervous system--specific HSN2 exon of WNK1 cause hereditary sensory neuropathy type II.
J Clin Invest. 2008 Jul;118(7):2496-505. doi: 10.1172/JCI34088.
Messaed C, Dion PA, Abu-Baker A, Rochefort D, Laganiere J, Brais B, Rouleau GA.
Soluble expanded PABPN1 promotes cell death in oculopharyngeal muscular dystrophy.
Neurobiol Dis. 2007 Jun; 26(3):546-57. Epub 2007 Feb 15.
Kharma N, Mognieh H, Yao J, Abu-baker A, Laganiere J, Rouleau GA, Cheriet M.
Automatic segmentation of cells from microscopic imagery using ellipse detection.
IET Image Processing, 2007 1(1), 39-47. Doi:10.1049/Iet-Ipr:20045262
Abu-Baker A, Laganiere S, Fan X, Laganiere J, Brais B, Rouleau GA.
Cytoplasmic targeting of mutant poly (A) binding protein nuclear 1 suppresses protein aggregation and toxicity in oculopharangeal muscular dystrophy.
Traffic 2005 Sep;6 (9): 766-79.
Dion P, Shanmugam V, Gaspar C, Messaed C, Meijer I, Toulouse A, Laganiere J, Roussel J, Rochefort D, Laganiere S, Allen C, Karpati G, Bouchard JP, Brais B, Rouleau GA.
Transgenic expression of an expanded (GCG) 13 repeat PABPN1 leads to weakness and coordination defects in mice.
Neurobiol Dis. 2005 Apr; 18(3):528-36.
Fan X, Messaed C, Dion P, Laganiere J, Brais B, Karpati G, Rouleau GA.
HnRNP A1 and A/B interaction with PABPN1 in oculopharyngeal muscular dystrophy.
Can J Neurol Sci. 2003 Aug; 30(3):244-51.
Abu-Baker A, Messaed C, Laganiere J, Gaspar C, Brais B, Rouleau GA.
Involvement of the ubiquitin-proteasome pathway and molecular chaperones in oculopharyngeal muscular dystrophy.
Hum Mol Genet. 2003 Oct 15; 12(20):2609-23. Epub 2003 Aug 27.
Fan X, Dion P, Laganiere J, Brais B, Rouleau GA.
Oligomerization of polyalanine expanded PABPN1 facilitates nuclear protein aggregation that is associated with cell death.
Hum Mol Genet. 2001 Oct 1; 10(21):2341-51.
Jannatipour M, Dion P, Khan S, Jindal H, Fan X, Laganiere J, Chishti AH, Rouleau GA.
Schwannomin isoform-1 interacts with syntenin via PDZ domains.
J Biol Chem. 2001 Aug 31; 276(35):33093-100. Epub 2001 Jun 29.
Pramatarova A, Laganiere J, Roussel J, Brisebois K, Rouleau GA.
Neuron-specific expression of mutant superoxide dismutase 1 in transgenic mice does not lead to motor impairment.
J Neurosci. 2001 May 15; 21(10):3369-74.
Shanmugam V, Dion P, Rochefort D, Laganiere J, Brais B, Rouleau GA.
PABP2 polyalanine tract expansion causes intranuclear inclusions in oculopharyngeal muscular dystrophy.
Ann Neurol. 2000 Nov; 48(5):798-802.
Gaspar C, Jannatipour M, Dion P, Laganiere J, Sequeiros J, Brais B, Rouleau GA.
CAG tract of MJD-1 may be prone to frameshifts causing polyalanine accumulation.
Hum Mol Genet. 2000 Aug 12; 9(13):1957-66.